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为探索中国的切割稻草饲料出口日本的消毒方法,用耐热性很强的水稻胡麻斑病菌Cochliobolus miyabeanus作为指标菌,在62、65、68、71和74℃下利用真空蒸汽法进行了不同时间的杀菌试验,得出了热致死TDT(thermal death time)曲线lg(Y)=10.4001-0.1447X,相关系数r=-0.9454。在此基础上,对外形尺寸为25cm×25cm×80cm、重20±1kg的商业切割饲料稻草捆按下述程序处理:抽真空至720mmHg,充100℃蒸汽5min,再抽真空至720mmHg,再充100℃蒸汽一定时间,最后抽真空15min。处理时在稻草捆中接种指标菌C.miyabeanus,草捆中心设置感温探头,根据温度和上移3倍标准差后的致死曲线lg(Y)=11.1843-0.1447X同步计算理论杀菌率。处理22～26min,理论上达到100％杀菌;所有接种菌全部灭活。处理前后稻草的品质未发生明显变化。
In order to explore the disinfection method of cutting straw forage in Japan for export to Japan, the thermostable Cochliobolus miyabeanus was used as an indicator bacteria at different times of vacuum evaporation at 62, 65, 68, 71 and 74 ℃ The results showed that the thermal death time curve lg (Y) = 10.4001-0.1447X, the correlation coefficient r = -0.9454. On this basis, on the commercial size of 25cm × 25cm × 80cm, weighing 20 ± 1kg commercial cutting straw bale processed according to the following procedure: vacuum to 720mmHg, 100 ℃ steam for 5min, and then evacuated to 720mmHg, and then recharge 100 ℃ steam for a certain time, the last vacuum 15min. Inoculation with rice straw breeder C.miyabeanus, the burettes set the temperature probe, according to the temperature and the upper limit of 3 times the standard deviation of the lethality curve lg (Y) = 11.1843-0.1447X synchronous calculation of the theoretical sterilization rate. Treatment 22 ~ 26min, in theory, to achieve 100% bactericidal; all inoculated bacteria inactivated. The quality of straw did not change significantly before and after treatment.