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本文通过测定大鼠血清γ-谷氨酰转肽酶(γ-GT),并与肝组织γ-GT组织化学和组织化学放射自显影相对照,研究了3′-甲基-4,4’-二甲基偶氮苯(3′-Me-DAB)诱发大鼠肝癌的癌变过程。由于非致癌化学性肝损害(D-半乳糖胺性肝炎)肝组织中γ-GT阴性,而γ-GT阳性的肝细胞结节具有较高的脱氧胸苷(~3HdT)标记指数(32.14％),与阴性结节相比(12.94％)差别显著(P<0.001),因此γ-GT作为肝癌及其癌前病变的标记是有一定的特异性。在大鼠诱癌的全过程中血清γ-GT含量始终高于正常水平,并波动呈马鞍形双峰曲线,结合对肝组织γ-GT组织化学的观察,对双峰曲线形成的病理基础和意义作了讨论,提出肝癌的组织发生至少可能有两种起源方式:(1)增生的卵圆细胞分化成肝细胞后,癌变形成分化差的肝细胞癌;(2)“幸存”的肝细胞发生增生,癌变成为分化较好的肝细胞癌。另外,对不典型增生的肝细胞显示γ-GT强阳性的意义也进行了讨论。
In this paper, we measured the serum γ-glutamyl transpeptidase (γ-GT) in rats and compared it with the liver tissue γ-GT histochemistry and histochemical autoradiography to study the 3′-methyl-4,4′ - Methylazobenzene (3’-Me-DAB) induces carcinogenesis of rat liver cancer. Due to non-carcinogenic chemical liver damage (D-galactosamine hepatitis) liver tissue γ-GT negative, and γ-GT positive hepatocyte nodules have a higher deoxythymidine (~3HdT) labeling index (32.14% ), compared with negative nodules (12.94%), the difference was significant (P<0.001). Therefore, γ-GT is a specific marker for liver cancer and its precancerous lesions. The serum γ-GT content was always higher than the normal level in the process of rat carcinogenesis, and it fluctuates to be a saddle-shaped bimodal curve. Combined with observation of the histochemistry of γ-GT in liver tissue, the pathological basis for the formation of double peak curve was The significance was discussed, suggesting that the histogenesis of hepatocellular carcinoma may have at least two ways of origin: (1) hepatocellular carcinoma with poorly metastasized cancer after differentiation of proliferating oval cells into hepatocytes; (2) “surviving” hepatocytes Hyperplasia occurs and the cancer becomes a well-differentiated hepatocellular carcinoma. In addition, the significance of displaying a strong positive γ-GT for atypical hyperplasia of hepatocytes has also been discussed.