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ANS(1-苯胺-8-萘磺酸)和DPH(1,6-二苯基-1,3,5,-己三烯)作为探针,用于检测γ射线对大鼠红细胞膜的作用。离体照射大鼠红细胞膜,随着剂量的增加(10—40 Gy),ANS结合红细胞膜的荧光强度降低。用DPH作为荧光探针,在整体实验中也得到了同样的结果,全身照射8.5 Gy之后的第1、3和5d,DPH结合大鼠红细胞膜的荧光强度下降,荧光偏振度增加。这些结果反映了照射使膜的流动性降低,可能是由于γ射线离体与整体照射诱导的膜不饱和脂肪酸的脂质过氧化及膜蛋白构象改变引起的。
ANS (1-aniline-8-naphthalenesulfonic acid) and DPH (1,6-diphenyl-1,3,5,-hexatriene) as probes for the detection of γ-rays on rat erythrocyte membranes . In vitro irradiation of rat erythrocyte membranes, with increasing doses (10-40 Gy), the fluorescence intensity of ANS-bound erythrocyte membranes decreased. Using DPH as a fluorescent probe, the same result was obtained in the whole experiment. On the 1st, 3rd, and 5th days after whole body irradiation of 8.5 Gy, the fluorescence intensity of the red blood cell membrane of DPH-bound rat decreased, and the degree of fluorescence polarization increased. These results reflect the decrease in fluidity of the membrane by irradiation, possibly due to the lipid peroxidation and membrane protein conformational changes of the unsaturated fatty acids induced by γ-rays in vitro and in vivo.