论文部分内容阅读
目的 探讨肺成纤维细胞 (FB)增殖与其合成胶原功能间的关系。方法 通过卟啉醇肉豆蔻酸乙酸乙酯 (PMA)和 1 (5 异喹啉基 ) 2 甲基哌嗪 (H 7)改变肺FB的增殖状态 ,观察肺FB数、细胞内DNA中3 H 胸腺嘧啶核苷 (3 H TdR)掺入量与细胞新合成蛋白质中3 H 脯氨酸 (3 H Pro)掺入量的关系。结果 2 0nmol/L的PMA作用 3d时 ,肺FB数 [(12 .2± 2 .3)× 10 4 个 /孔 ]和细胞内DNA中3 H TdR掺入量 [(1.89± 0 .0 9)× 10 3 Bq]分别较对照组 [(7.5±1.1)× 10 4 个 /孔、(1.6 7± 0 .16 )×10 3 Bq]明显增多 ,差异有显著性 (P <0 .0 1) ;但细胞分泌的蛋白质中3 H Pro掺入量 [(6 87.5± 2 0 0 .5 )Bq/ 10 5cells]比对照组 [(1181± 147.5 )Bq/ 10 5cells]却明显减少 ,差异有显著性 (P <0 .0 1)。5 0 μmol/L的H 7作用 3d时 ,肺FB数 [(4.2± 0 .8)× 10 4 个 /孔 ]和细胞内3 H TdR掺入量 [(0 .72±0 .13)× 10 3 Bq]分别较对照组 [(7.5± 1.1)× 10 4 个 /孔、(1.12± 0 .0 8)× 10 3 Bq]明显减少 ,差异有显著性 (P <0 .0 1) ,但3 H Pro掺入量 [(170 5 .5± 2 5 1.5 )Bq/ 10 5cells]比对照组 [(1181± 147.5 )Bq/ 10 5cells]明显增多 ,差异有显著性 (P <0 .0 1)。结论 肺FB合成胶原蛋白的功能与其增殖状态有关。肺
Objective To investigate the relationship between the proliferation of lung fibroblasts (FB) and the function of collagen synthesis. Methods The proliferative state of lung FB was changed by PMA and H 7, and the number of lung FB, the number of 3 H The relationship between incorporation of thymidine (3 H TdR) and 3H-proline (3H) incorporation into newly synthesized proteins in cells. Results When 3 nmol / L PMA was applied for 3 days, the number of lung FB [(12.2 ± 2.3) × 10 4 cells / well] and the amount of 3 H TdR incorporation in intracellular DNA [(1.89 ± 0.99 ) × 10 3 Bq] were significantly higher than those in the control group [(7.5 ± 1.1) × 10 4 cells / well, (1.6 7 ± 0.16) × 10 3 Bq] (P <0.01) ). However, the amount of 3 H Pro incorporation in the protein secreted by cells [(6 87.5 ± 200.5) Bq / 105cells] was significantly lower than that of the control group [(1181 ± 147.5) Bq / 105cells] Significance (P <0.01). The lung FB number [(4.2 ± 0.8) × 10 4 cells / well] and intracellular 3 H TdR incorporation [(0.72 ± 0.13) × 10 3 Bq] were significantly lower than those in the control group [(7.5 ± 1.1) × 10 4 cells / well, (1.12 ± 0.08) × 10 3 Bq] (P <0.01) However, the incorporation of 3 H Pro [(170 5 .5 ± 2 5 1.5) Bq / 105cells was significantly higher than that of the control group [(1181 ± 147.5) Bq / 105cells], with significant difference (P < 1). Conclusion The function of collagen synthesis in lung FB is related to its proliferation status. lung