论文部分内容阅读
目的通过二血管阻断加低血压法建立大鼠全脑缺血再灌注模型,观察δ阿片受体激动剂DADLE对大鼠肺水转运的作用,探讨其对全脑缺血再灌注肺损伤(ALI)的保护作用。方法健康雌性SD大鼠30只随机分为假手术组(Sham)、模型组(I/R)、DADLE处理组各10只。假手术组(Sham):只暴露血管而不夹闭;缺血再灌注组(I/R):采用改良的二血管阻断加低血压法建立全脑缺血再灌注模型,缺血15min后给予再灌注120min;DADLE处理组:于再灌注前经由左侧颈静注射DADLE5mg/kg,再灌注120min后,取右肺组织,常规HE染色观察其形态学改变,左肺叶组织湿质量/干质量(W/D)值。并与假手术组(Sham)、模型组(I/R)进行对照分析。结果成模后的肺脏表现为肺泡间隔增宽,毛细血管扩张充血,肺胞腔内及血管周围中性粒细胞浸润,气管壁部分上皮脱落,肺胞腔及气管腔均有浆液渗出。DADLE处理组肺脏充血减轻,中性粒细胞浸润有所减少。结论大鼠急性全脑缺血再灌注模型对肺有不同程度的损伤,DADLE可减少肺组织液体过量分泌,对ALI具有一定的保护作用。
OBJECTIVE: To establish a model of global cerebral ischemia-reperfusion in rats by occlusion of blood vessels and increase hypotension, and to observe the effect of DADLE, a δ opioid receptor agonist, on pulmonary water transport in rats and its effects on lung injury after global cerebral ischemia-reperfusion ALI) protection. Methods Thirty healthy female SD rats were randomly divided into sham operation group, model group (I / R) and DADLE treatment group (n = 10). Sham group: Exposed to only vessels without occlusion; Ischemia-reperfusion group (I / R): The model of global cerebral ischemia-reperfusion was established by modified two-vessel occlusion plus hypotension. After ischemia for 15 min The rats in DADLE group were given DADLE 5 mg / kg through left jugular vein before reperfusion and 120 minutes after reperfusion. The right lung tissues were taken out and the morphological changes were observed by routine HE staining. The wet / dry weight of left lung lobes (W / D) value. The control group was also compared with Sham group and model group (I / R). Results The lungs showed a widened alveolar septum, telangiectasia and hyperemia, infiltration of neutrophils in and around the lung cavity, epithelial detachment of the tracheal wall, and effusion of the lung cavity and trachea. DADLE treatment group reduced lung congestion, neutrophil infiltration decreased. Conclusion The rat model of acute global cerebral ischemia-reperfusion injury to varying degrees, DADLE can reduce lung fluid over-secretion of ALI has a protective effect.