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试验动物为150～200g豚鼠,在其左腹股沟处皮下注射0.2ml卡介苗(BCG),6周后,用有毒力的结核杆菌株经呼吸道攻击,杀死动物前24小时皮内注射0.1ml含100结核菌素单位的纯蛋白衍生物(PPD)进行皮试.用双玫瑰花环法测定带有IgG Fc受体的T细胞(Tγ细胞)或IgM Fc受体的T细胞(Tμ细胞)的比例.
The test animals were guinea pigs weighing 150-200 g and were subcutaneously injected with 0.2 ml BCG at their left groin. Six weeks later, they were challenged with the respiratory tract by a virulent Mycobacterium tuberculosis strain and intradermally injected with 0.1 ml Skin test was performed on pure protein derivative (PPD) of tuberculin units, and the ratio of T cells (Tγ cells) carrying IgG Fc receptor to T cells (Tγ cells) or IgM Fc receptors was determined by double rosette method.