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脑内的钙离子与针剌镇痛具有密切的关系。本文用钙离子选择电极研究了在少量脑样中测定pCa的方法,并观察了不同条件下小鼠脑匀浆中pCa的变化。采用本文介绍的测定pCa的方法,所得实验结果证明,不论在何种配体存在的体系中,pCa在1—8范围内对电极的响应都符合Nernst关系。离体脑制备亦是一个含有多种生物配体的复杂体系,因此,脑匀浆中的pCa应按Nernst响应的直线关系测定。在不同温度下和外加不同成分的脑匀浆中的pCa几乎都有一个先升高而后降低的过程,其差别仅在于pCa的变化速度快、慢不同。这些变化可能与脑匀浆中细胞逐渐死亡的生理生化过程有关。本文结果对于进一步研究中枢神经系统钙离子的生理作用和建立微量测定生物体内活性钙的方法具有一定的参考价值。
Calcium in the brain and acupuncture analgesia is closely related. In this paper, we used calcium ion selective electrode to study the method of determining pCa in a few brain samples, and observed the change of pCa in brain homogenate under different conditions. Using the method described in this article for the determination of pCa, the experimental results obtained demonstrate that the response of the pCa to the electrodes in the range of 1 to 8 is in line with the Nernst relationship regardless of the ligand present. Ex vivo brain preparation is also a complex system that contains multiple biological ligands. Therefore, pCa in brain homogenates should be measured as a linear relationship of Nernst response. In different temperature and different components of brain homogenates of pCa almost all have a first increase and then decrease the process, the only difference is that pCa changes rapidly, slowly different. These changes may be related to the gradual death of cells in brain homogenate physiological and biochemical processes. The results of this paper for the further study of the physiological role of central nervous system calcium and trace determination of bioactive calcium in vivo has some reference value.