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利用RNA-DNA杂交技术观察到:(1)在二乙基亚硝胺(DENA)诱发大鼠肝癌过程中,含有增生结节肝和肝细胞癌肝中c-Ha-ras、c-Ki-ras、N-ras和c-fos癌基因的表达都较正常肝高,而c-myc的表达无明显改变。(2)在两种不同诱发肝癌起始/促癌模型的起始阶段,c-myc的表达显著增高。在2-乙酰氨基芴作用下,N-ras表达的增高非常显著。c-myc和N-ras的表达似有协同作用。在促癌阶段,c-myc和N-ras的表达接近正常。(3)在起始和促癌过程中,c-fos的表达都显著降低,c-Ha-ras和c-Ki-ras表达变化不显著。对这些结果的意义作了初步讨论。
The RNA-DNA hybridization technique was used to observe: (1) c-Ha-ras, c-Ki- in the liver containing hyperplastic nodule liver and hepatocellular carcinoma during diethylnitrosamine (DENA)-induced liver cancer in rats The expression of ras, N-ras and c-fos oncogenes was higher than that of normal livers, but the expression of c-myc was not significantly changed. (2) The expression of c-myc was significantly increased in the initial stages of two different models of hepatoma initiation/promotion. Under the action of 2-acetylaminoindole, the increase of N-ras expression was very significant. The expression of c-myc and N-ras seems to have a synergistic effect. At the stage of promoting cancer, the expression of c-myc and N-ras is near normal. (3) In the process of initiation and promotion of cancer, the expression of c-fos was significantly decreased, and the expression of c-Ha-ras and c-Ki-ras was not significantly changed. A preliminary discussion of the significance of these results was made.