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目的 研究肺纤维化大鼠的血管内皮细胞生长因子 (VEGF)和转化生长因子 (TGF -β1)的合成和分布规律与血管新生的关系及其在肺纤维化发生、发展过程中的意义。方法 用免疫组织化学的原位杂交方法观察博莱霉素 (BLM )组和对照组在 3d、7d、14d、2 1d、2 8d时肺组织内VEGF和TGF -β1的合成和分布情况 ,并结合透射电镜及光学显微镜观察肺毛细血管的病理变化。 结果 博莱霉素大鼠VEGF与TGF -β1表达早期明显增强并且持续存在 ,两者呈平行增长 ,在肺内间质细胞的表达以 2 8d分布最广 ,其表达区域与血管新生及纤维组织增生区域相吻合。肺毛细血管内皮细胞早期见坏死、脱落、通透性增高 ,此后大量毛细血管新生 ,新生血管扭曲 ,通透性高 ,且可见血管内血栓形成。这种变化在 2 8d组仍然存在。结论 VEGF与TGF -β1的表达与血管新生密切相关 ,这两种因子的持续的高表达可能与新生血管损伤或功能障碍有关 ,进而在肺纤维化中起重要作用
Objective To investigate the relationship between the synthesis and distribution of vascular endothelial growth factor (VEGF) and transforming growth factor-β1 (TGF-β1) and angiogenesis in pulmonary fibrosis rats and their significance in the development and progression of pulmonary fibrosis. Methods The synthesis and distribution of VEGF and TGF-β1 in lung tissues of BLM group and control group at 3d, 7d, 14d, 21d and 28d were observed by immunohistochemical in situ hybridization Combined with transmission electron microscopy and optical microscopy to observe the pathological changes of pulmonary capillaries. Results The expression of VEGF and TGF-β1 in bleomycin rats increased significantly and persisted in the early stage, both of them showed parallel growth. The distribution of interstitial cells in lung was the most widely distributed in 28 days. The expression of VEGF and TGF- Area of hyperplasia coincide. Pulmonary capillary endothelial cells seen early necrosis, loss, increased permeability, since a large number of capillary angiogenesis, angiogenesis, hyperpermeability, and visible intravascular thrombosis. This change in the 28d group still exists. Conclusion The expression of VEGF and TGF-β1 is closely related to angiogenesis. The persistence and high expression of these two factors may be related to neovascular injury or dysfunction, which may play an important role in pulmonary fibrosis