目的了解医学生物学研究所健康人群抗甲型肝炎病毒抗体(抗HAV)水平,为制定防制措施提供科学依据。方法采集职工体检血清标本419份,采用酶联免疫吸附实验(ELISA法)对血清中抗HAV进行检测。结果职工HAV抗体总阳性率达83.3%;51~岁年龄组阳性率最高,为95.8%,22~30岁组阳性率最低,为73.1%;从事甲肝疫苗生产及相关人员抗HAV阳性率为83.3%,与生产无关人员抗HAV阳性率为82.7%;男性抗HAV阳性率为85.7%,女性抗HAV阳性率为81.0%。结论 HAV抗体阳性率随着年龄增长呈上升趋势,性别和工种无差别。建议对<30岁的年轻职工进行HAV疫苗接种,以提高这部分人员的整体免疫水平。 Objective To understand the level of anti-hepatitis A virus (anti-HAV) in healthy people in Institute of Medical Biology and provide a scientific basis for the development of control measures. Methods A total of 419 serum samples from employees were collected and tested for anti-HAV by enzyme-linked immunosorbent assay (ELISA). Results The positive rate of HAV antibody in workers was 83.3%. The highest positive rate was 95.8% in 51 ~ age group, the lowest was in 22 ~ 30 years old group (73.1%), the positive rate of anti-HAV in hepatitis A vaccine production was 83.3 %. The positive rate of anti-HAV was 82.7% in non-productive staff. The positive rate of anti-HAV in male was 85.7% and the positive rate of anti-HAV in female was 81.0%. Conclusions The positive rate of HAV antibody is increasing with age, and there is no difference between sex and type of work. It is recommended that young workers <30 years of age be vaccinated against HAV in order to increase the overall level of immunity of these individuals.