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目的 研究人支气管肺淋巴结尘细胞和大鼠腹膜腔巨噬细胞吞噬碳粒后的凋亡和bcl 2表达 ,探讨巨噬细胞凋亡与淋巴结结构变化之间的关系。 方法 取人支气管肺淋巴结 ,作石蜡切片和超薄切片 ,观察尘粒分布、凋亡尘细胞和组织结构变化。用TUNEL染色法和bcl 2抗体标记法观察淋巴结内尘细胞和碳粒处理后的巨噬细胞的凋亡变化和bcl 2表达。 结果 成人淋巴结巨噬细胞的尘粒明显沉积 ,淋巴组织减少 ,胶原纤维增生 ,血管密度增加。在超薄切片上 ,尘细胞的细胞核固缩 ,出现空泡。淋巴结尘细胞和巨噬细胞吞噬碳粒后 2 4h都出现TUNEL染色和bcl 2抗体标记阳性细胞。分解尘粒和碳粒活跃的巨噬细胞 ,bcl 2表达特别明显。 结论 尘粒沉积引起人支气管肺淋巴结巨噬细胞的凋亡和凋亡抑制基因bcl 2高表达 ,尘细胞凋亡与成人支气管肺淋巴结的结构变化有关。
Objective To study the apoptosis and expression of bcl-2 after phagocytosis of macrophages from peritoneal macrophages of bronchoalveolar lacteal cells and rat peritoneal macrophages, and to explore the relationship between macrophage apoptosis and the changes of lymph node structure. Methods Bronchial lung lymph nodes were taken for paraffin sections and ultrathin sections to observe the distribution of dust particles and the changes of apoptotic dust cells and tissue structure. TUNEL staining and bcl 2 antibody labeling were used to observe the changes of apoptosis and the expression of bcl 2 in the lymph node and carbon granule-treated macrophages. Results Significant deposition of dust particles in adult lymph node macrophages, decreased lymphoid tissue, hyperplasia of collagen fibers, and increased vascular density. In the ultra-thin slices, the nucleus of dust cells shrinks and vacuoles appear. Lymph node dust cells and macrophages after engulfing carbon particles 24 h appeared TUNEL staining and bcl 2 antibody-positive cells. Decomposition of dust particles and activated carbon macrophages, bcl 2 expression was particularly evident. Conclusion Dust deposition induces the apoptosis of human bronchial pulmonary lymph node macrophages and the high expression of apoptosis-inhibiting gene bcl 2. The apoptosis of dust cells is related to the structural changes of bronchopulmonary lymph nodes in adults.