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病毒必须依赖宿主细胞的酶和代谢系统才能生存和进行复制。组织培养法由于其本身的许多优点已成为理想的病毒培养系统。组织培养技术的发展为病毒学提供了很好的工具和方法,它已广泛应用于病毒性疾病的诊断、疫苗生产、抗病毒药物的研究以及病毒学基础理论的研究,如病毒复制过程、病毒免疫和病毒生物化学等。 从培养类型来看,组织培养可分为组织块培养、原代细胞培养、次代细胞培养、传代细胞系、传代细胞株和器管培养等。
Viruses must rely on the host cell’s enzymes and metabolic systems to survive and replicate. Tissue culture has become an ideal viral culture system because of many of its own advantages. The development of tissue culture technology provides a good tool and method for virology. It has been widely used in the diagnosis of viral diseases, vaccine production, antiviral drug research and the basic theory of virology, such as virus replication process, virus Immune and viral biochemistry. From the type of culture point of view, tissue culture can be divided into tissue culture, primary cell culture, the second generation of cell culture, subculture cell lines, cell lines and tube culture and so on.