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在暗間隔0.16秒的3.3×10~(-4)秒閃光下,比較了PMS,Vit.K,FMN及Fe(CN)_6~≡光合磷酸化反应系統的每閃最高产量以及温度的影响,并观察邻二氮杂菲对这些反应系統每閃最高产量抑制的差异,以及在閃时延长时,它对閃光产量抑制程度的变化,得到結果如下: (1)在短閃光下,PMS,Vit.K,FMN及Fe(CN)_6~≡光合磷酸化反应系統的每閃最高产量都极接近,温度的影响也不太显著。(2)邻二氮杂菲对Vit.K,FMN及Fe(CN)_6~≡非循环光合磷酸化反应系統的每閃最高产量的50%抑制濃度都在0.96×10~(-5)M/左近,而对PMS系統的每閃最高产量的50%抑制濃度則力4.5×10~(-5)M。(3)邻二氮杂菲对PMS及Vit.K这两种类型的光合磷酸化反应系統在連續弱光下反应速度的50%抑制濃度与对这两反应系統的每閃最高产量的50%抑制濃度相同,即分别为4.5×10~(-5)M及0.95×10~(-5)M;同样温度(5℃)而光强增加到100000米烛光,则对这两反应系统反应速度的50%抑制浓度提高到依次为14.0×10~(-5)M及2.3×10~(-5)M。如果再把温度从5℃提升到30℃,则50%抑制浓度更提高到25.0×10~(-5)M及4.0×10~(-5)M。(4) 邻二氮杂菲对Vit.K系统的短闪光的每闪最高产量的抑制程度不受闪光光强影响,然而当闪光的闪时增加时,抑制剂对闪光产量的抑制程度则随闪时增加而减少。由此推论,邻二氮杂菲的抑制部位与光合磷酸化反应的光化作用中心有关,增加闪光闪时,或在连续光下增加光强,或在饱和光下提高温度都使光化作用中心增加重复利用而使抑制剂的抑制作用减弱。循环(PMS)及非循环(Vit.K,FMN,Fe(CN)_6~≡)光合磷酸化反应系统的闪光产量相同,而对邻二氮杂菲的敏感程度却相差很大的现象,可以用放氧及磷酸化各有一光化作用中心解释之。非循环光合磷酸化反应系统包括有放氧及磷酸化两个,而循环光合磷酸化反应系统则仅需磷酸化一个光化作用中心。
Under the flash of 3.3 × 10 ~ (-4) seconds with dark interval of 0.16 second, the maximum flash per output and temperature of PMS, Vit.K, FMN and Fe (CN) _6 ~ ≡ photophosphorylation reaction systems were compared. And observed phenanthroline inhibition of these systems for each flash maximum yield difference, as well as in the flash extended, its suppression of flash output changes, the results are as follows: (1) in a short flash, PMS, Vit .K, FMN and Fe (CN) _6 ~ ≡Photosynthetic phosphorylation reaction system, the maximum yield per flash are very close, the impact of temperature is not too significant. (2) The 50% inhibitory concentrations of phenanthroline on the highest yield per florescence of Vit.K, FMN and Fe (CN) _6 ~ ≡ acyclic photosynthesis phosphorylation system are all 0.96 × 10 ~ (-5) M / Left, while the 50% inhibitory concentration for the highest yield of PMS system per flash is 4.5 × 10 -5 M. (3) The 50% inhibitory concentration of phenanthroline on both PMS and Vit.K photophosphorylation systems under continuous weak light and 50% of the maximal yield per flash for both reaction systems (5 × 10 -5 M) and 0.95 × 10 -5 M respectively at the same temperature (5 ℃) and the light intensity increased to 100000 m candle light, then the response speed of these two reaction systems The 50% inhibitory concentration increased to 14.0 × 10 ~ (-5) M and 2.3 × 10 ~ (-5) M in turn. If the temperature is raised from 5 ° C to 30 ° C, the 50% inhibitory concentration is further increased to 25.0 × 10 -5 M and 4.0 × 10 -5 M. (4) The inhibition of phenanthrene phenanthrene to the highest output per flash of short flash of Vit.K system is not affected by flash light intensity, however, the suppression of flash output by the inhibitor increases with flash flash flash Flash increases and decreases. From this, we deduced that the phenanthroline phenanthrene inhibition site is related to the photoactivation center of photosynthetic phosphorylation reaction. The increase of flash flash, the increase of light intensity in continuous light or the increase of temperature in saturated light all make photochemical reaction Center to increase the reuse and inhibition of the inhibitor weakened. The photosynthetic phosphorylation reaction system of PMS and non-cyclic (Vit.K, FMN, Fe (CN) _6 ~ ≡) has the same flash output, but the sensitivity to phenanthroline is quite different. With oxygen and phosphorylation each have a photochemical center explained. Acyclic photosynthetic phosphorylation reaction system includes two oxygen and phosphorylation, and photosynthetic phosphorylation cycle system only needs to phosphorylate a photochemical center.