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目的研究鼠李糖乳杆菌和植物乳杆菌等8种常见益生菌对阪崎肠杆菌的拮抗作用。方法采用牛津杯法测定益生菌耗尽上清对阪崎肠杆菌的抑菌圈,获得对阪崎肠杆菌具有较强抑菌能力的鼠李糖乳杆菌和植物乳杆菌;采用混合培养法对2株益生菌与阪崎肠杆菌的拮抗竞争能力进行测试。结果 8种益生菌耗尽上清均能抑制阪崎肠杆菌,其抑菌能力具有热稳定性且依赖于酸性pH环境。阪崎肠杆菌(107CFU/mL)与鼠李糖乳杆菌(108CFU/mL或109CFU/mL)共孵育至24 h,其活菌量开始逐渐下降,至120 h孵育结束下降到105CFU/mL;菌量比为1:10的阪崎肠杆菌与植物乳杆菌共孵育至24 h,其活菌量开始逐渐下降,菌量比为1:100时则提前至8 h,至120 h孵育结束活菌量均下降到102CFU/mL。结论鼠李糖乳杆菌和植物乳杆菌均能有效地竞争拮抗阪崎肠杆菌。
Objective To study the antagonistic effects of eight common probiotics such as Lactobacillus rhamnosus and Lactobacillus plantarum on Enterobacter sakazakii. Methods The inhibitory zone of probiotic bacteria-depleted supernatant against Enterobacter sakazakii was determined by the Oxford cup method, and the Lactobacillus rhamnosus and Lactobacillus plantarum which had strong antibacterial activity against Enterobacter sakazakii were obtained. The mixed culture method Two strains of probiotics and Enterobacter sakazakii competitive antagonism were tested. Results All of the eight kinds of probiotics depleted supernatant could inhibit Enterobacter sakazakii. The bacteriostasis ability was thermostable and dependent on acidic pH environment. Enterobacter sakazakii (107CFU / mL) was incubated with Lactobacillus rhamnosus (108CFU / mL or 109CFU / mL) for 24 hours, and the viable cell count began to decrease gradually to 105CFU / mL after 120 hours incubation. The ratio of 1:10 Enterobacter sakazakii and Lactobacillus plantarum co-incubated to 24 h, the viable count began to decline, when the bacteria volume ratio of 1: 100, then advance to 8 h, to 120 h incubation end of live bacteria The amount decreased to 102CFU / mL. Conclusion Both Lactobacillus rhamnosus and Lactobacillus plantarum can effectively compete against Enterobacter sakazakii.