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超低浓度分析试样的测定已日显重要。特别在生物样品的临床检测方面的需求更显迫切。作者所进行的一系列研究中应用了对单个抗原—抗体键敏感的光学捕获技术,对蛋白质抗原的检测下限可达毫微微克分子浓度。应用激光光束的光箝装置被用于捕获及远程调控介电粒子,如细胞及其它生物体,由此而引起的动量改变可导致折射率的变化而被检测。光箝原理与免疫技术的结合可用于免疫检测。免疫技术中的竞争耦合置换被用于检测溶液中的微量抗原。BSA抗原被用作检测实例。实验结果表明:检测下限可达1.45×10~(15)mol/L,亦即66个BSA分子。
Determination of ultra-low concentration of the sample has become increasingly important. In particular, the need for clinical testing of biological samples is even more pressing. A series of studies carried out by the authors applied an optical capture technique that is sensitive to a single antigen-antibody bond, with a detection limit of up to femtograms of protein antigens. Optical clamp devices using laser beams are used to capture and remotely control the growth of dielectric particles, such as cells and other organisms, and the resultant changes in momentum can cause changes in refractive index to be detected. The combination of optical clamp principle and immunological technique can be used for immunoassay. Competitive coupled permutations in immunological techniques are used to detect trace antigens in solution. BSA antigen was used as a detection example. The experimental results show that the detection limit can reach 1.45 × 10 ~ (15) mol / L, which means 66 BSA molecules.