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目的 :探讨不同运动训练方式和补剂对耗竭运动后恢复期骨骼肌糖原生成素 (GN)基因表达的影响 ,并观察GN基因和α -actin基因表达之间是否关联。方法 :以成年雄性SD大鼠为研究对象 ,采用正交设计法安排实验 ,研究耐力游泳训练、间歇高强度训练、肌酸、谷氨酰胺 4因素对上述指标的效应 ,实验期 2周。结果 :在糖原耗竭运动后恢复期 ,耐力训练和间歇高强度训练大鼠骨骼肌GN基因表达增高 ,运动后 2 4小时呈显著性差异 (P <0 . 0 5 ) ;补充肌酸或谷氨酰胺对GN基因表达未见明显影响。运动后骨骼肌α -actin基因表达各研究因素间无显著差异 ;GN基因和α -actin基因表达之间无时效关联。结论 :耐力训练和间歇高强度训练大鼠在糖原耗竭运动后恢复期骨骼肌GN基因表达增高 ,补充肌酸和谷氨酰胺对运动后GN基因表达无显著影响 ,运动后骨骼肌GN基因与α -actin基因表达不同步。
Objective: To investigate the effect of different exercise training methods and supplements on the gene expression of glycogenin (GN) in the recovery period after exhaustive exercise, and to observe whether GN gene and α-actin gene expression are related. Methods: Adult male Sprague-Dawley rats were used as research objects. The orthogonal design was used to study the effects of endurance swimming training, intermittent high-intensity training, creatine and glutamine on the above indexes. The experimental period was 2 weeks. Results: During the recovery period of glycogen depletion exercise, the GN gene expression of skeletal muscle in endurance training and intermittent high-intensity training rats increased significantly after 24 hours of exercise (P <0. 05) The amino acid amide had no obvious effect on GN gene expression. There was no significant difference in the expression of α-actin gene between skeletal muscle after exercise and no correlation between GN gene and α-actin gene expression. CONCLUSIONS: Endurance training and intermittent high-intensity training increased the expression of GN gene in skeletal muscle during recovery from glycogen depletion exercise. Creatine supplementation and glutamine supplementation did not significantly affect GN expression after exercise. After exercise, GN α-actin gene expression is not synchronized.