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为探索黄连总生物碱及小檗碱对嗜水气单胞菌的抑菌活性及作用机制,测定了黄连总生物碱和小檗碱对嗜水气单胞菌的最小抑菌浓度、膜通透性、膜流动性、膜蛋白构象及毒力因子的影响。结果显示,黄连总生物碱和小檗碱对嗜水气单胞菌均有抑菌活性,其最小抑菌浓度分别为62.5,125 mg·L-1。黄连总生物碱和小檗碱能增加膜脂流动性,改变膜蛋白构象,并使菌体膜通透性分别增加24.52%,19.66%。此外,黄连总生物碱和小檗碱显著性地抑制菌体外毒素的溶血活性及aer A和hly A mRNA的表达(P<0.05,P<0.01),同时降低嗜水气单胞菌内毒素的分泌量和Lpx C mRNA的表达(P<0.05,P<0.01)。结果表明,黄连总生物碱和小檗碱对嗜水气单胞菌的抑菌作用可能与细胞膜损伤有关,通过改变膜脂流动性和膜蛋白构象使菌体膜通透性增加从而抑制了细菌生长;同时降低了细菌的毒力因子的分泌,使得菌体的致病性降低。
In order to explore the antibacterial activity and mechanism of total alkaloids of berberine and berberine against Aeromonas hydrophila, the minimum inhibitory concentrations (MICs) of Coptidis alkaloids and berberine against Aeromonas hydrophila were determined. Permeability, membrane fluidity, membrane protein conformation and virulence factors. The results showed that Coptis alkaloids and berberine have Aeromonas hydrophila antibacterial activity, the minimum inhibitory concentration of 62.5,125 mg · L-1. Coptis alkaloids and berberine can increase the fluidity of membrane lipid, change the conformation of membrane protein, and make membrane permeability increase by 24.52% and 19.66% respectively. In addition, Coptis alkaloids and berberine significantly inhibited the hemolysis activity of exotoxin and the expression of aer A and hly A mRNA (P <0.05, P <0.01), and at the same time decreased the levels of Aeromonas hydrophila endotoxin The secretion and the expression of Lpx C mRNA (P <0.05, P <0.01). The results showed that the antibacterial effect of berberine alkaloids and berberine on Aeromonas hydrophila may be related to cell membrane damage. By changing the membrane fluidity and membrane protein conformation, Growth; at the same time reduce the secretion of bacterial virulence factors, making the bacterial pathogenicity decreased.