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为了确定盐胁迫下大麦籽粒产量和萌发期生长指标与小孢子培养阶段盐胁迫下愈伤组织产量之间是否存在一致性,以2份大麦品种为供试材料,进行了以下3项研究:(1)诱导培养基中NaCl含量对小孢子培养愈伤组织产量的影响;(2)萌发液中NaCl含量对大麦种子萌发期生长指标的影响;(3)NaCl胁迫处理对大麦单株产量的影响。结果表明,诱导培养基中NaCl含量提高可降低小孢子培养愈伤组织产量,但2份品种的降幅存在明显的差异;萌发液中NaCl含量提高可降低种子发芽率、主根长度和胚芽鞘长度,2份品种间降幅上也存在明显的差异;盆栽条件下NaCl胁迫处理的大麦单株产量明显低于无NaCl的对照,2份品种间有差异。NaCl胁迫下,2份供试材料小孢子培养愈伤组织产量的相对值与种子萌发期的发芽率、主根长度和胚芽鞘长度的相对值以及单株产量的相对值存在着一定的相关,说明供试品种的耐盐性在小孢子水平与植株水平上是一致的。
In order to determine whether there is consistency between the grain yield and the germination index of barley under salt stress and the callus production under salt stress during the microspore culture stage, two barley varieties were used as materials for the following three studies: 1) Effect of NaCl concentration on callus production in microspore culture; (2) Effect of NaCl content in germination solution on growth index of barley seed during germination; (3) Effect of NaCl stress on yield per plant of barley . The results showed that the increase of NaCl concentration in induction medium could reduce the callus production of microspore culture, but there was a significant difference in the decline of two cultivars. The increase of NaCl in germination solution could reduce the germination rate, length of main root and coleoptile length, There was also a significant difference in the decline between the two cultivars. The yield per plant of barley under NaCl stress was significantly lower than that of NaCl-free control under pot culture. There were differences among the two cultivars. Under NaCl stress, the relative values of callus production of two microspore culture materials were related to the germination rate, the relative length of main root length and coleoptile length, and the relative value of single plant yield, indicating that The salt tolerance of the tested varieties is consistent with the microspore level and plant level.