目的 :在国内首次建立乙醛所致大鼠肝星状细胞增殖的离体模型。方法 :通过大鼠肝星状细胞的分离、培养 ,取传一代星状细胞作为对象 ,选择乙醛 17.5 μmM为基础浓度 ,以 5、10、5 0、15 0、2 0 0、2 5 0倍递增浓度作为刺激因子 ,用MTT及Giemsa染色法观察乙醛对星状细胞的作用。结果 :875 μmM乙醛为最佳浓度 ,可使星状细胞增殖率和存活率均达到最大。Giemsa染色该浓度细胞增殖最为活跃。结论 :单纯用乙醛作用于肝星状细胞可以形成肝星状细胞的增殖离体模型 ,适用于以后的多种相关实验。 OBJECTIVE: To establish an in vitro model of rat hepatic stellate cell proliferation induced by acetaldehyde for the first time in China. Methods: Rat hepatic stellate cells were isolated, cultured, and passaged into the first generation of stellate cells as the target. Acetaldehyde 17.5 μmM was selected as the base concentration, and 5,10,5 0,15 0,2 0 0,2 5 0 Fold increase concentration as stimulating factor, the effect of acetaldehyde on stellate cells was observed by MTT and Giemsa staining. Results: 875 μmM acetaldehyde was the best concentration, which could maximize the proliferation rate and survival rate of stellate cells. Giemsa stain the concentration of the most active cell proliferation. CONCLUSIONS: Acetaldehyde alone acts on hepatic stellate cells to form an in vitro model of proliferation of hepatic stellate cells, which is suitable for a variety of related experiments in the future.