以胞嘧啶核苷为目标分子,合成对其有专一性识别的分子印迹聚合物(MIP)。在酸性条件下,利用HCl-KMn O4-HCHO发光体系,结合流动注射化学发光(FI-CL)分析方法,创建对胞嘧啶核苷测定有特异性识别的MIP-FI-CL分析方法,其线性范围为8×10-5~1×10-3mol/L,检出限为4×10-5mol/L。利用此方法测定复杂样品中胞嘧啶核苷的含量,结果令人满意。 Cytosine was used as the target molecule to synthesize molecularly imprinted polymer (MIP). Under acidic conditions, a MIP-FI-CL analytical method that specifically recognizes cytosine nucleosides is created using the HCl-KMn O4-HCHO luminescence system in combination with the flow-injection chemiluminescence (FI-CL) The range of 8 × 10-5 ~ 1 × 10-3mol / L, the detection limit of 4 × 10-5mol / L. Using this method for the determination of cytidine in complex samples, the results are satisfactory.