论文部分内容阅读
目的了解2009年宁波市甲3亚型流感病毒的流行特征及血凝素基因HA1区域的变异情况。方法全年度每周从流感监测哨点医院收集流感样病例标本进行病毒分离,获得的甲3亚型流感毒株按不同时间、地点进行血凝素基因HA1区核苷酸序列测定并推导出其氨基酸序列,然后进行基因特性分析。结果所选取的15株甲3亚型流感病毒HA1区域核苷酸序列长度均为987 bp,编码329个氨基酸;2009年上半年的流行株与2008年秋季的流行株在该区域有7个氨基酸位点存在差异,2009年下半年的流行株与2009上半年又存在10个氨基酸位点的差异。上半年与下半年的流行株在种系发生树上形成两个分支,为不同性状的流行株。结论 2009年上半年及下半年宁波市甲3亚型流感流行株与2008年相比,在血凝素基因HA1区域都发生了较为明显的改变,且上半年与下半年的2个流行株之间血凝素基因的差异也较大。说明2009年宁波市流行的甲3亚型流感病毒变异较为频繁。
Objective To understand the epidemiological characteristics of influenza A (H3N1) virus in Ningbo in 2009 and the variation of HA1 in hemagglutinin gene. Methods The samples of influenza-like illness were collected from the influenza surveillance sentinel hospitals every week for virus isolation. The obtained influenza A (3) virus strains were sequenced according to different time and place, and the deduced amino acid sequence of HA1 Amino acid sequence, and then gene characterization. Results The nucleotide sequences of the HA1 region of the 15 strains of influenza A virus subtype H3 were all 987 bp, encoding a protein of 329 amino acids. In the first half of 2009 and in the fall of 2008, the prevalence of influenza virus HA1 region was 7 amino acids There were differences between the two sites in the second half of 2009 and the first half of 2009 there are 10 amino acid differences. In the first half and the second half of the epidemic strains in the phylogenetic tree formed two branches, for the different traits of the epidemic strains. Conclusion In the first half of 2009 and the second half of 2009, the pandemic strains of influenza A-3 subunit in Ningbo showed a significant change in HA1 region of hemagglutinin gene compared with that in 2008, and two epidemic strains in the first half and the second half of the year Hemagglutinin gene differences are also larger. This shows that the epidemic of influenza A virus in Ningbo in 2009 is more frequent.