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采用套式PCR对含HIV—1膜蛋白基因的B亚型质粒和HIV—1感染者外周血单核细胞(PBMC)进行env基因部分片断的扩增,套式PCR可使常规PCR的敏感性提高100至1000倍,常规PCR只扩增出12份PBMC中的2份样品,套式PCR则扩增出全部样品中HIV—1膜蛋白基因片断.
A nested-PCR method was used to amplify the env gene fragment from the subtype B plasmid containing HIV-1 membrane protein and the peripheral blood mononuclear cells (PBMC) from HIV-1 infected patients. Nested PCR can make the sensitivity of conventional PCR Two hundred samples of 12 PBMCs were amplified by conventional PCR, and the HIV-1 membrane protein gene fragments of all the samples were amplified by nested PCR.