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将用ＣＰＶ免疫的ＢＡＬＢ／ｃ小鼠脾细胞与ＳＰＶ２／０细胞在聚乙二醇作用下融合，用ＨＩ法筛选，有限稀释法克隆三次，得到６株分泌抗ＣＰＶＭｃＡｂ的杂交瘤细胞株，其中４株分泌ＩｇＧ１，２株分泌ＩｇＭ。将杂交瘤细胞注射ＢＡＬＢ／ｃ小鼠诱生腹水，摸索出了大量生产腹水的最佳实验条件。采用ＣＰＶ ＭｃＡｂ研制触ＣＰＶ快速诊断试剂盒，检测４７份病犬粪样，在３０ｍｉｎ即可肉眼判定结果，与ＨＡ法和常规ＥＬＩＳＡ相比，具有简便、快速、特异之优点。应用ＣＰＶＭｃＡｂ治疗ＣＰＶ发病犬２８７只，治愈率高达９７．２１％，显著高于其他疗法的治疗效果，治疗机理除对体内病毒的中和作用外，有可能促进机体的主动免疫反应。在对４６只幼犬进行ＣＰＶ疫苗免疫时，使用ＣＰＶＭｃＡｂ的２３只，其免疫成功率为１００％，而未使用ＣＰＶＭｃＡｂ的２３只对照犬，其免疫成功率仅为７８．３％，表明ＣＰＶ ＭｃＡｂ在疫苗免疫中可起积极作用。
The BALB / c mouse spleen cells immunized with CPV were fused with SPV2 / 0 cells under the action of polyethylene glycol, screened by HI method and cloned by limiting dilution three times to obtain six hybridoma cell strains secreting anti-CPVMcAb 4 strains secreted IgG1 and 2 strains secreted IgM. BALB / c mice were injected with hybridoma cells to induce ascites, and the optimal experimental conditions for mass production of ascites were explored. CPV McAb was used to develop a rapid CPV diagnostic kit. 47 cases of canine dung samples were detected, and the result of naked eye examination was determined within 30 minutes. Compared with HA and conventional ELISA, the method was simple, rapid and specific. The application of CPVMcAb in the treatment of CPV dogs 287, the cure rate as high as 97.21%, significantly higher than the therapeutic effect of other therapies, in addition to the mechanism of treatment of the virus in vivo neutralization, it may promote the body’s active immune response. Twenty-three puppies CPV vaccine immunized with CPVMcAb had an immunization success rate of 100%, whereas 23 control dogs without CPVMcAb had a vaccine success rate of only 78.3%, indicating that CPV McAb Vaccination can play a positive role.