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目的:研究低温处理对半夏悬浮培养细胞同步化的影响,找出适合半夏细胞同步化条件。方法:以三叶半夏疏松愈伤组织为材料,采用正交试验设计方法研究低温处理对半夏悬浮培养细胞同步化的影响,并对所得数据进行极差分析和方差分析。结果:低温处理后,细胞分裂指数均有不同程度的提高,其中,温度为8℃时细胞分裂指数大于其他2组温度处理后细胞的分裂指数,而在8℃低温条件下,处理24 h,恢复36 h,同步化效果最好,分裂指数可达11.3%。结论:低温处理可以明显影响半夏悬浮培养细胞的同步化,有效提高其分裂指数。
OBJECTIVE: To study the effect of low temperature treatment on the synchronization of cells in suspension culture of Pinellia ternata, and to find out the conditions suitable for the synchronization of Pinellia ternata cells. Methods: The loose callus of Trefoilopsis pilosula was used as material. The effects of chilling treatment on the synchronization of suspension culture cells of Pinellia ternata were studied by orthogonal design. The data were analyzed by range and variance analysis. Results: The cell division index increased with different degrees after low temperature treatment. The cell division index was higher than that of the other two groups under the condition of 8 ℃ and 24 hours, Recovery 36 h, the best synchronization, split index up to 11.3%. Conclusion: Low temperature treatment can significantly affect the synchronization of suspended culture cells in Pinellia ternata, and effectively improve the division index.