应用荷移络合物理论,采用可见分光光度法测定青霉素钾(钠)盐含量。青霉素与四氯苯醌结合呈紫色,在560nm处呈最大吸收。并在O.1-O.7mg/ml范围内符合Beer′s定律。所绘制的吸收度-浓度标准曲线的r值极接近1。方法简便、准确、灵敏度高。 Using charge-transfer complex theory, the content of penicillin potassium (sodium) salt was determined by visible spectrophotometry. Penicillin and chloranil combined with a purple, maximum absorption at 560nm. Beer’s law was obeyed in the range of 0.1-1.7 mg / ml. The absorbance-concentration standard curve plotted for r has an extremely close value of 1. The method is simple, accurate and sensitive.