外源基因直接导入技术是单子叶植物基因工程研究的核心内容,近年来其发展十分迅速。本文较为系统地介绍了这一技术的各种新方法及其特点,并分析比较了这些方法的优缺点和应用前景,同时还介绍了利用这些方法在禾谷类作物转基因研究中所取得的成果。 Direct introduction of exogenous gene technology is the core content of monocot genetic engineering, its development is very rapid in recent years. This article systematically introduces various new methods and characteristics of this technology, and analyzes the advantages and disadvantages of these methods and their application prospects. At the same time, it also introduces the results of using these methods in the study of the transgenic crops of cereals.