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分析了茶树插穗生根过程中内源激素含量及相互比值动态变化。结果表明:3种主要激素在不同生根率品种间的变化趋势基本一致。插穗刚脱离母体时,IAA和ABA含量大幅度下降,而ZR略有下降;在愈伤组织形成及根原基孕育期IAA,ZR含量迅速增加,而ABA降低并保持较低浓度;生根后IAA,ZR下降而ABA迅速增加恢复至正常生长水平。在生根过程中起主要调节作用的是IAA和ABA。离体时不同生根率品种间插穗IAA/ABA值与其插穗生根率成正相关,可以作为插穗生根能力的主要指标。
The dynamic changes of endogenous hormones and mutual ratio during the rooting of tea cuttings were analyzed. The results showed that the trends of the three main hormones in different rooting rate varieties were basically the same. The content of IAA and ABA decreased sharply while the ZR decreased slightly when the cuttings were detached from the maternal. The content of IAA and ZR increased rapidly during the period of callus formation and root primordium, while the ABA decreased and remained at a low level. The IAA, ZR decreased and ABA rapidly increased to normal growth. The main regulatory roles in rooting are IAA and ABA. The IAA / ABA value of intercalants with different rooting percentages was positively correlated with the rooting rate of cuttings in vitro, which could be used as the main index of rooting ability of cuttings.