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应用荧光光谱法研究了水溶液中喹诺酮抗菌素氧氟沙星、环丙沙星与过氧化氢酶分子间的结合反应。结果表明：药物对过氧化氢酶的内源荧光有较强的猝灭作用，形成复合物所产生的静态猝灭是引起过氧化氢酶荧光猝灭的主要原因。进一步依据荧光猝灭结果确定了药物－酶复合物的形成常数和结合位点数。
Fluorescence spectroscopy was used to study the binding of quinolone antibioticloxacin, ciprofloxacin and catalase in aqueous solution. The results show that the drug has a strong quenching effect on the endogenous fluorescence of catalase, and the static quenching caused by the complex formation is the main reason for the fluorescence quenching of catalase. Based on the fluorescence quenching results, the formation constants and the number of binding sites of the drug-enzyme complex were also determined.