本文用SDS聚丙烯酰胺凝胶电泳法和聚丙烯酰胺凝胶等电聚焦法,测定了莱阳生化制药厂生产的猪胸腺素的分子量和等电点。一、分子量凝胶浓度为10％,交联度为2.7％,电泳缓冲液为0.1M磷酸-SDS缓冲液,pH7.0,上槽接阴极,下槽接阳极,电泳在每支胶管恒定8毫安电流下进行,约4～5小时,示踪染料移动至胶管下端 In this paper, SDS polyacrylamide gel electrophoresis and polyacrylamide gel isoelectric focusing were used to determine the molecular weight and isoelectric point of porcine thymosin produced by Laiyang Biochemical Pharmaceutical Factory. First, the molecular weight gel concentration of 10%, the degree of crosslinking of 2.7%, electrophoresis buffer 0.1M phosphate-SDS buffer, pH7.0, connected to the upper tank cathode, the tank connected to the anode, electrophoresis in each hose constant 8 MA current, about 4 ~ 5 hours, the tracer dye moved to the lower end of the hose