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目的:检测宫颈鳞癌组织中P-选择素蛋白及mRNA的表达情况,探讨其在宫颈鳞癌发生发展中的作用。方法:选取2011年1月~2014年4月承德市多家医院手术切除的宫颈鳞癌标本60例为病例组,宫颈正常组织40例为对照组。采用免疫组化方法与逆转录-聚合酶链式反应(RT-PCR)技术联合检测两组宫颈组织中P-选择素蛋白及mRNA的表达情况。结果:病例组P-选择素蛋白及mRNA的表达阳性率(63.3%和58.3%)均高于对照组(35.0%和27.5%),差异有统计学意义(P<0.05);病例组P-选择素蛋白及mRNA的表达均与分化程度、p TNM分期、淋巴结转移和浸润深度相关(P<0.05),而与年龄无关(P>0.05)。结论:宫颈鳞癌组织中P-选择素蛋白及mRNA的表达与宫颈鳞癌的发生、发展紧密相关。应用免疫组化方法与RT-PCR技术联合检测宫颈鳞癌组织中P-选择素蛋白及mRNA可评估宫颈鳞癌浸润和转移的能力,并为预后的预测提供理论依据。
Objective: To detect the expression of P-selectin and mRNA in cervical squamous cell carcinoma and to investigate the role of P-selectin in the development and progression of cervical squamous cell carcinoma. Methods: From January 2011 to April 2014, 60 cases of cervical squamous cell carcinoma resected in many hospitals of Chengde City were selected as case group and 40 cases of normal cervical tissue as control group. The expressions of P-selectin and mRNA in cervical tissue were detected by immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR). Results: The positive rates of P-selectin and mRNA expression in case group were significantly higher than those in control group (63.3% vs 58.3%, 35.0% vs 27.5%, P <0.05) The expressions of selectin and mRNA were correlated with the degree of differentiation, p TNM stage, lymph node metastasis and depth of invasion (P <0.05), but not with age (P> 0.05). Conclusion: The expression of P-selectin and mRNA in cervical squamous cell carcinoma is closely related to the occurrence and development of cervical squamous cell carcinoma. The combination of immunohistochemistry and RT-PCR in the detection of P-selectin and mRNA in cervical squamous cell carcinoma can evaluate the ability of invasion and metastasis of cervical squamous cell carcinoma, and provide a theoretical basis for the prediction of prognosis.