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在电化学检测液相色谱中已广泛采用双电极检测以提高选择性和灵敏度,利用维生素B_2的可逆电化学反应,本文采用串联双电极还原-氧化检测维生素B_2。使用一实验室装置的伏安分析计,循环伏安法采用三电极的电池系统:玻璃碳工作电极、银-氯化银参比电极和铂辅助电极。色谱系统包括Waters510型泵、U 6 K进样阀、481型可变波长检测器。进样量20μl,检测波长254 nm,流动相流速每分钟
In the electrochemical detection of liquid chromatography has been widely used to improve the selectivity and sensitivity of the two-electrode detection, the use of vitamin B 2 reversible electrochemical reaction, this article series of two-electrode reduction - oxidation of vitamin B_2. Using a voltammetric analyzer for a lab setup, cyclic voltammetry uses a three-electrode battery system: a glassy carbon working electrode, a silver-silver chloride reference electrode, and a platinum auxiliary electrode. The chromatography system includes a Waters 510 pump, a U 6 K injection valve, and a 481 variable wavelength detector. Injection volume 20μl, detection wavelength 254 nm, mobile phase flow rate per minute