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目的了解外环境水体标本在庆大霉素平板上的菌群分布及优势菌的耐药谱,研究提高霍乱弧菌检出率的方法。方法采集闽江水体用庆大平板分离培养,用K-B法检测20种抗菌药物对优势菌的敏感性;选择痢特灵,将不含霍乱弧菌的水样一代以及二代增菌液作为稀释液,对不同型别的霍乱弧菌进行倍比稀释,观察其在庆大平皿上的生长情况。结果 330份标本共检出优势菌151株,其中79.5%为气单胞菌。151株优势菌对阿米卡星、诺氟沙星、环丙沙星等9种抗菌素的敏感率达80%以上。在一代增菌液为稀释介质的生长试验中,当痢特灵的浓度≥4μg/mL时,不同型别的霍乱弧菌能检测到的最低菌浓度均有一定的提高;而以二代增菌液为稀释介质时,痢特灵的浓度需≥8μg/mL或16μg/mL。结论在庆大霉素琼脂平皿中加入不同浓度的痢特灵,在一定程度上能提高霍乱弧菌的检出率。
Objective To understand the flora distribution of outer water samples in gentamycin plate and the drug resistance spectrum of dominant bacteria, and to study the methods to improve the detection rate of V. cholerae. Methods The Minjiang River water samples were collected and cultured with Qingda plate, and the susceptibility of 20 antibacterials to predominant bacteria was detected by KB method. The furazolidone-free water samples and the second-generation bacteriostasis were selected as dilution Liquid, Vibrio cholerae of different types of dilution ratio to observe its growth in Qingda plate. Results A total of 151 dominant bacteria were detected in 330 samples, of which 79.5% were Aeromonas. 151 strains of dominant bacteria on amikacin, norfloxacin, ciprofloxacin and other 9 kinds of antibiotics sensitivity rate of more than 80%. In the growth test of generation medium with dilution medium, when the concentration of furazolidone was ≥4μg / mL, the lowest concentration of bacteria detected by different types of Vibrio cholerae all increased to a certain extent. When the bacterial liquid is diluted media, the concentration of furazolidone should be ≥8μg / mL or 16μg / mL. Conclusion Adding different concentrations of furazolidone to gentamycin agar plate can improve the detection rate of V. cholerae to a certain extent.