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异黄酮是大豆生长过程中形成的一类次级代谢产物,是大豆主要的功能活性成分之一。异黄酮还原酶(isoflavone reductase,IFR)是异黄酮分解途径的关键酶之一,在调控异黄酮含量及成分方面起着重要作用。本研究基于大豆全基因组测序数据,利用生物信息学方法鉴定了大豆异黄酮还原酶基因家族成员,并对其基因的序列特征、结构特征和遗传多样性进行了分析。结果表明:大豆异黄酮还原酶(GmIFR)家族含有17个成员,蛋白序列介于191(GmIFR07)和376(GmIFR11)个氨基酸之间,序列相似性为19.59%(Gm IFR07和GmIFR11)~98.43%(Gm IFR12和GmIFR17),结构分析表明这些基因内含子数目差异较大,2~6个不等,不均匀的分布在大豆的1、4、6、9、11、12和16号染色体上。
Isoflavones are a kind of secondary metabolites formed during the growth of soybean and are one of the main functional active ingredients of soybean. Isoflavone reductase (isoflavone reductase, IFR) is one of the key enzymes in the isoflavone degradation pathway and plays an important role in the regulation of isoflavone content and composition. Based on the genome-wide sequencing data of soybean, the members of soybean isoflavone reductase gene family were identified by using bioinformatics methods. The sequence, structural characteristics and genetic diversity of these genes were analyzed. The results showed that the GmIFR family consisted of 17 members with a protein sequence between 191 (GmIFR07) and 376 (GmIFR11) amino acids with a sequence similarity of 19.59% (Gm IFR07 and GmIFR11) to 98.43% (Gm IFR12 and GmIFR17). The structural analysis showed that the number of introns in these genes differed greatly from 2 to 6, which were unevenly distributed on chromosomes 1, 4, 6, 9, 11, 12 and 16 of soybean .