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本应用报告开发并验证了一种同时检测四种β2-激动剂残留物的方法,包括特布他林、沙丁胺醇、克仑特罗和福莫特罗。分析物经过液-液萃取(LLE)和固相萃取(SPE)纯化后,由正离子多反应监测模式下的液相色谱耦合电喷雾串联质谱(LC-ESI-MS/MS)进行定量。该方法为检测猪肉中四种β2-激动剂提供了亚ng/g水平的检测限(LOD),以及0.25至5ng/g的动态校准范围。总回收率为78%至101%,相对标准偏差(RSD)为1.8%至7.2%。
This application note has developed and validated a method for the simultaneous detection of four β2-agonist residues, including terbutaline, salbutamol, clenbuterol and formoterol. The analytes were purified by liquid-liquid extraction (LLE) and solid-phase extraction (SPE) and quantified by liquid chromatography-tandem electrospray ionization tandem mass spectrometry (LC-ESI-MS / MS) in positive ion multiple reaction monitoring mode. The method provides a limit of detection (LOD) for sub-ng / g levels of four beta 2-agonists in pork and a dynamic calibration range of 0.25 to 5 ng / g. The total recoveries ranged from 78% to 101% with relative standard deviations (RSDs) of 1.8% to 7.2%.