对20例年龄1.5～78岁、病程1～24年的喉乳头状瘤活检标本,用Southern氏涂抹杂混(blot hybridization)法分析了人类乳头状瘤病毒DNA的存在。所有被检标本均含有乳头状瘤病毒DNA片断。取自2例活动期病人非受累部位的4份标本和取自缓解期病人的8份标本亦含有病毒DNA。这些结果解释了即使是喉乳头状瘤病长期缓解后的频繁复发性临床类型。作者们得出两点重要结论:首先,乳头状瘤 Twenty patients with laryngeal papilloma biopsies ranging in age from 1.5 to 78 years and from 1 to 24 years of age were analyzed for the presence of human papilloma virus DNA using southern blot hybridization. All examined specimens contained papillomavirus DNA fragments. Four specimens from two non-affected areas of active patients and eight specimens from remission patients also contained viral DNA. These results explain the frequent recurrent clinical pattern even after long-term remission of laryngeal papillomatosis. The authors draw two important conclusions: First, papilloma