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52只Wistar大白鼠针刺致脑干机械性损伤，LSAB法显示胶质原纤维酸性蛋白阳性（GFAP+）星形胶质细胞和波形蛋白阳性（VIM+）星形胶质细胞改变。结果发现，脑干损伤15分钟组GFAP+星形胶质细胞出现肿胀；1～3小时组中脑腹侧等处细胞数目增多，6小时组细胞数目明显减少，24～48小时组恢复正常水平，1～6小时组细胞轻度肥大，染色稍强；24～96小时组创周GFAP+星形胶质细胞数目明显增多，中度肥大，染色明显增强；10～15天组细胞数目、肥大和染色强度达高峰。正常对照组大鼠脑干除脑桥腹侧近脚间窝处外，其余部位无VIM+星形胶质细胞；脑干损伤48小时组创周出现VIM+星形胶质细胞，96小时组细胞数目、染色强度和肥大程度达高峰，10～15天组下降，胞突弯曲变细。死后脑干损伤组GFAP+星形胶质细胞无改变，创缘及创周无VIM+星形胶质细胞出现。研究表明，星形胶质细胞可作为推断脑干损伤的客观指标。
Fifty-two Wistar rats were acupunctured to induce mechanical damage to the brain stem. LSAB staining showed changes in glial fibrillary acidic protein (GFAP +) astrocytes and vimentin-positive (VIM +) astrocytes. The results showed that GFAP + astrocytes became swollen at 15 minutes in brainstem injury group. The numbers of cells in ventral midbrain increased in 1 ~ 3 hours group, the number of cells in 6 hours group decreased significantly, and returned to the normal level in 24-48 hours group, The number of GFAP + astrocytes significantly increased from 24 to 96 hours in the group of moderate to severe hypertrophy and the staining increased significantly. The number of cells in the group of 10 to 15 days, the number of hypertrophy and staining The intensity of the peak. Normal control rats brain brainstem in addition to ventral patellar fossa near the other places without VIM + astrocytes; brain injury 48 h group VIM + astrocytes were found in the week, the number of cells 96 hours group, Dyeing intensity and hypertrophy reached the peak, 10-15 days group decreased, cell curve bends. There was no change in GFAP + astrocytes after death in brain stem injury group, and there was no VIM + astrocytes in wound edge and wound healing. Studies have shown that astrocytes can be used as objective indicators to infer brain damage.