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用 PEL A为材料包裹伤寒沙门菌荚膜多糖 ,制成缓释微球疫苗 ,分别以口服及皮下注射的方式免疫BAL B/ C小鼠 ,分别在第 2 ,4 ,8周取血 ,收集唾液 ,用 RIA方法检测血清中 Ig G抗体及唾液中 s Ig A抗体效价。第 8周腹腔注射伤寒沙门菌活菌攻击各组免疫动物 ,计算免疫动物保护率。单剂微球疫苗皮下注射比常规液体疫苗更有效地诱导全身免疫应答 (P<0 .0 5 ) ,而口服微球疫苗组和微球疫苗皮下注射组均可诱导较强粘膜免疫 (P<0 .0 5 )。动物保护力实验表明 ,口服微球疫苗组免疫保护率 4 0 % ,微球疫苗皮下注射组免疫保护率为 10 0 % ,常规液体疫苗免疫保护率为 6 0 %。缓释伤寒荚膜多糖微球疫苗具有在体内释放速度慢 ,免疫效果好等优越性
BALB / C mice were immunized BALB / C mice by oral and subcutaneous injection with PEL A as the material to encapsulate the capsular polysaccharide of Salmonella typhimurium. The blood was harvested at the 2nd, 4th, and 8th week, respectively. Saliva, IgA antibody in serum and s Ig A antibody titer in saliva were detected by RIA method. Week 8 of intraperitoneal Salmonella typhi inoculation challenge animals immunized groups to calculate the rate of protection of immunized animals. Single-agent microspheres vaccines induced systemic immune responses more effectively than conventional liquid vaccines (P <0.05), whereas both oral microspheres vaccine and microspheres vaccine subcutaneously induced strong mucosal immunity (P < 0 .0 5). Animal protection experiments showed that the immune protection rate of oral microsphere vaccine was 40%, the microspheres vaccination subcutaneous injection group was 100%, and the conventional liquid vaccine was 60%. Sustained release typhoid capsular polysaccharide microspheres vaccine has the advantages of slow release in the body and good immune effect