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利用低温灌注技术，电镜技术和生化检测技术，对低温保存人肝脏肝窦内皮细胞的损伤进行了研究。结果显示：低温保存时不仅肝实质细胞受到损伤，而且肝窦内皮细胞发生更严重的损伤，随着保存时间延长，嘌呤核苷磷酸化酶（PNP）逐渐升高。透射电镜和扫描电镜显示：人肝脏低温保存后，肝窦内皮细胞可出现变性肿胀，脏窦内大泡形成，随着保存时间延长，肝窦内皮细胞出现变性坏死导致肝窦阻塞。肝窦内皮细胞损伤后可导致肝脏微循环系统连续性和完整性的破坏，这可能是肝脏微循环障碍和移植肝功能衰竭的主要原因。
Low temperature perfusion, electron microscopy and biochemical detection techniques were used to study the damage of hepatic sinusoid endothelial cells in cryopreserved human liver. The results showed that not only hepatic parenchymal cells were injured during hypothermic storage, but also the hepatic sinusoidal endothelial cells harmed more seriously. The purine nucleoside phosphorylase (PNP) increased gradually with the prolongation of storage time. Transmission electron microscopy and scanning electron microscopy showed that the hepatic sinusoidal endothelial cells could be denatured and swollen, and bullae formed in the visceral sinus after cryopreservation. With the prolongation of preservation time, the sinusoidal endothelial cells denatured and necrotic resulted in obstruction of hepatic sinusoids. After sinusoidal endothelial cell injury can lead to damage to the continuity and integrity of the liver microcirculation system, which may be the main cause of liver microcirculation disorders and liver transplantation failure.