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目的:采用超高效液相色谱联用飞行时间质谱(UPLC-Q-TOF/MS)代谢组学方法分析江油附子与安县附子的化学成分差异。方法:利用UPLC-Q-TOF/MS技术对江油附子与安县附子化学成分群进行采集,采用Mass Hunter B.05.00软件与Metabo Analyst 3.0软件进行数据过滤与标准化处理,经SIMCA-P 13.0软件进行多元统计分析,发现并鉴定出两者区分的显著性差异化合物。结果:经偏最小二乘法判别分析筛选得到16个差异化合物;新乌头碱、乌头碱、展花乌头宁、塔拉乌头胺、苯甲酰新乌头原碱、苯甲酰次乌头原碱、脱水苯甲酰新乌头原碱及新乌头原碱在安县附子中含量较江油附子更高,而尼奥林、附子灵、次乌头碱、塔拉他定、卡米查林、附子亭、宋果灵在江油附子中含量更高,这种差异的形成与两地的栽培条件差异有关。结论:安县附子的毒性更强、镇痛抗炎活性更强,江油附子的强心活性可能更强。
OBJECTIVE: To analyze the chemical constituents of aconite in Jiangyou Fuzi and Anxian by using UPLC-Q-TOF / MS metabolomics. Methods: The chemical constituents of Aconitum kaempferolum were collected by UPLC-Q-TOF / MS. Mass Hunter B.05.00 software and Metabo Analyst 3.0 software were used for data filtering and standardization. The data were analyzed by SIMCA-P 13.0 software Statistical analysis identified and identified significant differences between the two compounds. Results: 16 differential compounds were obtained by partial least squares analysis and discriminant analysis; mesaconitine, aconitine, aconitine, taraconamine, benzoyl mesaconitine, Aconitine alkaloids, dehydrobenzoyl new aconitum base and capillaris alkali in Anxian aconite content higher than the Jiangyoufuzi, and Niorelin, aconitine, hypaconitine, talatatidine, card Michalin, aconite kiosk, Song Guoling in Jiangyou aconite content higher, the formation of this difference with the cultivation of two places on the differences. CONCLUSION: The aconite of An County is more toxic and has more analgesic and anti-inflammatory activity, and its cardiac activity may be stronger.