[目的]分离并鉴定1株产紫杉醇的曼地亚红豆杉内的内生真菌。[方法]从曼地亚红豆杉树皮内表皮中分离得到32株内生真菌,并通过高效液相色谱法检测其发酵产物。[结果]筛选获得1株可以产紫杉醇的内生真菌M57,其紫杉醇产量为45～50μg/L,并通过对M57菌落的形态学观察以及18S rDNA序列分析初步将其鉴定为根霉属(Rhizopus)真菌。[结论]该菌株的发现为微生物发酵法生产紫杉醇提供了具有潜在应用价值新的菌种。 [Objective] The research aimed to isolate and identify endophytic fungi in a taxol-producing Taxus mairei. [Method] Thirty - two endophytic fungi were isolated from the inner epidermis of the bark of Taxus chinensis and their fermentation products were detected by high performance liquid chromatography. [Result] One paclitaxel-producing endophytic fungus M57 was screened and its paclitaxel yield was 45-50 μg / L. The M57 colony was identified as Rhizopus (Rhizopus) by morphological observation and 18S rDNA sequence analysis ) Fungi. [Conclusion] The discovery of this strain provided a new strain with potential application for the production of paclitaxel by microbial fermentation.