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目的:观察大鼠角膜新生血管(cornealneovasculariza-tion,CNV)形成过程中环氧合酶-2(COX-2)的表达情况及与CNV的关系,探讨COX-2抑制剂Celecoxib对CNV的抑制作用。方法:利用免疫组织化学方法、RT-PCR法检测碱烧伤后各个时期COX-2和VEGF蛋白、mRNA在角膜各层中的分布,并对其进行半定量。比较实验组和对照组各个时期COX-2和VEGF蛋白和mRNA表达量的差别,并对其进行统计学分析,了解COX-2与VEGF的相关性。结果:①活化的COX-2和VEGF蛋白、mRNA的表达在角膜新生血管的形成中均有一动态变化。②VEGF的表达区域和COX-2表达的部位高度一致。③实验II组和实验Ⅲ组COX-2和VEGF蛋白、mRNA表达量与对照组的差别有显著性(P<0.05),实验I组与对照组的差别无显著性(P>0.05)。实验组和对照组COX-2与VEGF的表达呈正相关。结论:①COX-2在炎症性角膜新生血管形成过程中表达上调,其调控VEGF的表达,在角膜新生血管的形成过程中起着重要作用。②Celecoxib能抑制COX-2的表达,有效的抑制角膜新生血管的形成。
OBJECTIVE: To observe the expression of cyclooxygenase-2 (COX-2) in corneal neovasculariza- tion (CNV) and its relationship with CNV in rats and to explore the inhibitory effect of COX-2 inhibitor Celecoxib on CNV . Methods: Immunohistochemistry and RT-PCR were used to detect the distribution of COX-2 and VEGF protein and mRNA in different layers of cornea at various stages after alkali burn. The difference of the protein and mRNA expression of COX-2 and VEGF between the experimental group and the control group was statistically analyzed, and the correlation between COX-2 and VEGF was analyzed. Results: ① The expression of activated COX-2 and VEGF protein and mRNA both had a dynamic change in the formation of corneal neovascularization. ② The expression area of VEGF was highly consistent with the expression of COX-2. (3) There was a significant difference between COX-2 and VEGF protein and mRNA expression in experimental group II and III (P <0.05). There was no significant difference between experimental group I and control group (P> 0.05). There was a positive correlation between the expression of COX-2 and VEGF in experimental group and control group. CONCLUSION: ①COX-2 is up-regulated in the process of inflammatory corneal neovascularization, which regulates the expression of VEGF and plays an important role in the formation of corneal neovascularization. ②Celecoxib can inhibit the expression of COX-2 and effectively inhibit the formation of corneal neovascularization.