论文部分内容阅读
在叶片中合成由FLOWERING LOCUS T(FT)编码的成花素蛋白通过韧皮部运输到顶端分生组织(SAM),与b ZIP转录因子FD相互作用形成复合物,激活花分生组织身份基因的表达,从而调节植物开花和其他的一些生物学过程。本研究以棉花全基因组数据库为基础,发掘并得到18个棉花FD基因的序列并确定它们在各基因组染色体上的位置。生物信息学分析显示,棉花FD蛋白质分子量在15.69~28.24 k D之间,理论等电点在9.24~10.38之间,是一种非分泌性蛋白;亚细胞定位预测显示,棉花FD蛋白分布于细胞核上,是典型的核蛋白;氨基酸序列分析表明,棉花FD是一种亲水性蛋白,还存在着糖基化位点和磷酸化位点以及4个保守基序;进化分析表明,棉花FD1和FD2与葡萄Vv FD基因亲缘关系最近。组织表达分析表明,陆地棉10个Gh FD基因在不同组织中具有表达特征多样性,Gh FD5-D在纤维中的表达量最高,其余Gh FD基因均在SAM中的表达量最高,不同的表达特征表明它们可能具有不同的功能。这些结果为进一步解析棉花FD基因的功能和作用机理积累了有价值的资料。
Synthesis of Flavonetin encoded by FLOWERING LOCUS T (FT) in leaves. Transporting to the apical meristem (SAM) via the phloem, interaction with the b ZIP transcription factor FD forms a complex that activates the expression of flower meristem identity genes , Thus regulating plant flowering and some other biological processes. In this study, based on the genome-wide database of cotton, the sequences of 18 cotton FD genes were found and their positions on each genome were determined. Bioinformatics analysis showed that the molecular weight of cotton FD protein was between 15.69 and 28.24 kD and the theoretical isoelectric point was between 9.24 and 10.38, which was a non-secreted protein. The subcellular location prediction showed that cotton FD protein distributed in the nucleus Amino acid sequence analysis showed that cotton FD is a hydrophilic protein with glycosylation sites and phosphorylation sites as well as four conserved motifs. Phylogenetic analysis showed that cotton FD1 and FD2 has the closest genetic relationship with grape Vv FD gene. Tissue expression analysis showed that 10 Gh FD genes of Gossypium hirsutum had characteristic expression diversity in different tissues. Gh FD5-D had the highest expression in the fiber, and the remaining Gh FD genes had the highest expression in SAM with different expression Features indicate that they may have different functions. These results provide valuable data for further analysis of the function and mechanism of cotton FD gene.