[目的]探讨内生细菌Bacillussp.对菠菜枯萎病的防病作用。[方法]就内生菌Bacillussp.对菠菜枯萎病菌平板抑制作用、不同浓度内生菌对菠菜枯萎病的防治效果及内生菌在菠菜根部的定殖位点进行研究。[结果]结果表明,浓度为1×107cfu/ml时,Bacillus sp.对菠菜枯萎病防治效果最好,且内生菌定殖在菠菜根部木质部导管中。[结论]从菠菜根部筛选出的内生细菌Bacillussp.对菠菜枯萎病有的较好防治效果,可以利用此开发具有潜在生防价值的生物制剂。 [Objective] The research aimed to study the anti-disease effect of endophytic bacterium Bacillussp on Fusarium oxysporum f. [Method] With regard to the inhibition effect of endophyte Bacillussp to Fusarium oxysporum f. Sp., The effect of different concentration of endophyte on the control of Fusarium wilt of spinach and the colonization site of endophyte in the root of spinach. [Result] The results showed that Bacillus sp. Had the best control effect on Fusarium oxysporum f. Sp. At the concentration of 1 × 107 cfu / ml, and the endophytes colonized the xylem in the root of spinach. [Conclusion] Bacillussp, an endophytic bacterium screened from the root of spinach, had good control effect on the blight of spinach and could be used to develop biological agents with potential biocontrol value.