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用流式细胞术(FCM)检测59例慢性萎缩性胃炎(CAG)、74例胃癌细胞 DNA倍性、S期细胞比率(SPF)和增殖指数(PI),同时以18例正常胃粘膜作为对照。结果:单纯 CAG4.35%检出异倍体,CAG 伴肠化生和不典型增生及胃癌的异倍体检出率分别为25%和66.23%,差异有显著性(P<0.05)。从对照组到单纯 CAG、CAG 伴肠化生不典型增生至胃癌,SPF 和 PI递增,各组之间有显著差异(P<0.05或 P<0.01)。FCM 细胞 DNA 倍性、SPF 和 PI 分析有助于胃癌癌前病变监测。
Flow cytometry (FCM) was used to detect DNA ploidy, S phase cell ratio (SPF), and proliferation index (PI) in 59 cases of chronic atrophic gastritis (CAG) and 74 cases of gastric cancer, and 18 cases of normal gastric mucosa were used as controls. . RESULTS: The detection of aneuploidy in CAG4.35% alone, the detection rate of CAG with intestinal metaplasia, dysplasia, and gastric cancer were 25% and 66.23%, respectively, with significant difference (P<0.05). From control group to simple CAG, CAG with dysplasia of intestinal metaplasia to gastric cancer, SPF and PI increased, there was significant difference between each group (P<0.05 or P<0.01). DNA ploidy, SPF and PI analysis of FCM cells are helpful for the monitoring of precancerous lesions of gastric cancer.