植物激素的水平和分布对植物的生长和发育起着重要的作用。本文构建了不同启动子调控下的ipt基因的嵌合表达质粒,在土壤农杆菌的介导下转化烟草得到了再生植株。Southern杂交,PCR扩增,Northern杂交和NPTⅡ基因表达的检測表明ipt基因已整合在转基因烟草的基因组中,并有mRNA的转录和NPTⅡ基因的表达,转基因烟草在生长发育上有侧芽发达,节间缩短,根系粗壮和叶衰老迟缓等现象。 The level and distribution of plant hormones play an important role in plant growth and development. In this paper, chimeric expression plasmids of ipt gene under different promoters were constructed, and regenerated plants were transformed by tobacco mediated by Agrobacterium tumefaciens. Southern hybridization, PCR amplification, Northern hybridization and NPTⅡ gene expression analysis showed that the ipt gene has been integrated into the genome of transgenic tobacco with mRNA transcription and expression of NPTⅡ gene. Transgenic tobacco has lateral buds developed in growth and development, Shortened, stout roots and leaf aging slow and so on.