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目的建立高效液相法同时测定撤热柴胡饮中连翘苷和黄芩苷含量的方法。方法采用phenomenex-Cl8柱(250×4.6mm,5μm),以甲醇-水-磷酸(47∶53∶0.2)为流动相等度洗脱,检测波长为280 nm,流速为1.0 mL/min,柱温为30℃,进样量为10μL。结果在选定的色谱条件下,测定了连翘苷、黄芩苷两种组分的含量。连翘苷在0.0252~0.2520μg范围内呈良好的线性关系,平均回收率为98.65%(RSD=0.65%,n=6),黄芩苷在0.04832~0.4832μg范围内呈良好的线性关系,平均回收率为97.07%(RSD=1.24%,n=6)。结论该法快速,简便易行,结果可靠,专属性强,可作为该制剂的质量监控手段。
OBJECTIVE To establish a method for the simultaneous determination of forsythin and baicalin in Radix Bupleuri Decoction by high performance liquid chromatography. Methods Phenomenex-Cl8 column (250 × 4.6 mm, 5 μm) was used for the mobile phase elution of methanol-water-phosphoric acid (47:53:0.2). The detection wavelength was 280 nm and the flow rate was 1.0 mL / For 30 ℃, the injection volume is 10μL. Results Under the selected chromatographic conditions, the contents of forsythin and baicalin were determined. Forsythin had a good linear relationship in the range of 0.0252 ~ 0.2520μg, the average recovery was 98.65% (RSD = 0.65%, n = 6), and baicalin showed a good linear relationship in the range of 0.04832 ~ 0.4832μg with average recovery The rate was 97.07% (RSD = 1.24%, n = 6). Conclusion The method is rapid, simple and easy to operate with reliable results and high specificity. It can be used as a quality control measure for this preparation.