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目的了解不同方法在鼠疫监测现场中的应用效果,掌握检测方法的可行性、可操作性及对鼠疫监测的影响因素,评价方法间的一致性,制订适于现场应用的有效监测机制。方法对广西隆林、西林县鼠疫监测点收集的鼠脏器同时进行分离培养、反向间接血凝试验(RIHA)、胶体金免疫层析试验(RGICA)、酶联免疫吸附试验(ELISA)、聚合酶链反应(F1-Pla-PCR)。结果分离培养、RIHA、RGICA、ELISA、PCR检测鼠脏器中鼠疫菌F1抗原均为阴性,5种方法间一致性比较Kappa值等于0,呈轻度一致。结论新技术胶体金免疫层析试验、酶联免疫吸附试验、聚合酶链反应具有操作简便易学、特异、快速、敏感等特点,适宜在鼠疫监测现场中应用。
OBJECTIVE To understand the application effects of different methods in the plague surveillance field, to master the feasibility and operability of the detection methods and the factors affecting the surveillance of plague, to evaluate the consistency of the methods and to formulate an effective monitoring mechanism suitable for field application. Methods The rat organs collected from the plague surveillance sites in Longlin and Xilin County of Guangxi Province were isolated and cultured at the same time. RIHA, RGICA, ELISA, Polymerase chain reaction (F1-Pla-PCR). Results The results of isolation and culture, RIHA, RGICA, ELISA and PCR were all negative for the detection of Y. pestis F1 antigen. The consistency of the five methods was equal to 0, showing a slight agreement. Conclusion The new technology of colloidal gold immunochromatographic assay, enzyme-linked immunosorbent assay, polymerase chain reaction has the characteristics of easy to learn, specific, rapid and sensitive, and is suitable for the application in the plague monitoring field.