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通过喂饲大鼠高脂饮食建立高脂血症动物模型,在此基础上采用反复脑缺血再灌注方法诱发学习、记忆功能障碍,从行为学方面观察了复圣散对拟痴呆大鼠学习、记忆能力的改善作用,并进一步利用神经生化和受体研究方法考察了该药改善智能的中枢机制。结果:复圣散可明显减少术后大鼠水迷宫实验的错误次数,并缩短游全程时间(P<0.05)。脑生化及受体结合实验表明,脑缺血再灌术后大鼠EAAs释放异常,而NMDA受体持续激活,经复圣散预防和治疗性给药后,大鼠NMDA受体活性与同期模型组比较分别下降了34.94％和34.22％(P均<0.01),同时发现用药后CAT的活性明显提高(P<0.05或P<0.01)。揭示中药复圣散对脑缺血性损伤所致的学习记忆功能障碍具有显著的改善作用。
The animal model of hyperlipidemia was established by feeding a high-fat diet in rats, and learning and memory dysfunction were induced by repeated cerebral ischemic reperfusion on the basis of this. From the perspective of behavior, Fusheng San was studied on rats with dementia. The improvement of memory ability, and further use of neural biochemical and receptor research methods to examine the drug’s central mechanism for improving intelligence. Results: Fusheng San can significantly reduce the number of errors in the water maze experiment after operation and shorten the travel time (P<0.05). Brain biochemistry and receptor binding experiments showed that after cerebral ischemia and reperfusion, the release of EAAs was abnormal in rats, while NMDA receptors were continuously activated, and the activity of NMDA receptors in rats was compared with that of synchronous models after preventive and therapeutic administration of Fusheng San. The group had a decrease of 34.94% and 34.22% respectively (P <0.01), and the activity of CAT was significantly increased after treatment (P <0.05 or P <0.01). It is revealed that Fusheng San has a significant improvement in learning and memory dysfunction caused by cerebral ischemic injury.