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经济合作与发展组织(organization for economic cooperation and development,OECD)从1998年开始修订和制定了一些筛选潜在内分泌干扰物的试验方法,并于2009年颁布了最新一批的内分泌干扰物筛选试验方法,内分泌干扰物的测试方法主要有几个水平,包括整体动物、组织器官、细胞、基因等。本文主要介绍大鼠(抗)雄性性征Hershberger短期生物筛选试验和稳定转染的人雌激素受体α转录活性试验,分别采用整体动物和细胞进行体内和体外检测化学物质的类激素作用,为判定该化学物质是否属于内分泌干扰物提供一定依据。
Since 1998, the Organization for Economic Cooperation and Development (OECD) has revised and developed some test methods for screening potential endocrine disruptors. In 2009, the latest batch of endocrine disruptor screening test methods were promulgated. Endocrine disruptors test several major levels, including the whole animal, tissues and organs, cells, genes and so on. This article mainly introduces the short-term biological screening test of Hershberger male rats and the stable transfection of human estrogen receptor alpha transcriptional activity test, using whole animals and cells for in vivo and in vitro detection of chemical hormones, Determine whether the chemical belongs to endocrine disruptors to provide a basis.