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本文着重研究广西普通野生稻在离体条件下,诱导率,分化率与原分布地、形态类型及其优良特性的关系。试验共采用了28个编号,接种了近63000个花药,获得了1447块愈伤组织。野生与半野生类型的诱导率分别为0-15.31%和0-8.5%。18个编号出了白苗529株,4个编号出了绿菌22株。野生与半野生类型白化苗分化率为18.75-71.43%和9.09-58.82%;绿苗分化率为2.56-8.22%和5.88-13.33%。鉴定了部分编号的再生植株,染色体数n=12,2n=24。分化绿苗的材料(编号1、10、19、26)具有抗稻瘟病、高蛋白质含量、耐寒性强和抗白叶枯病的特性,这些材料为水稻育种提供了宝贵的遗传资源。
This article focuses on the relationship between the induction rate, differentiation rate and the original distribution, morphological type and its excellent characteristics of wild Oryza rufipogon in Guangxi under in vitro conditions. The experiment used a total of 28 numbers, inoculated nearly 63,000 anthers, obtained 1447 callus. Induction rates of wild and semi-wild type were 0-15.31% and 0-8.5%, respectively. There were 529 white seedlings in 18 numbers and 22 green ones in 4 numbers. The differentiation rates of wild and semi-wild type albino seedlings were 18.75-71.43% and 9.09-58.82%. The differentiation rates of green plantlets were 2.56-8.22% and 5.88-13.33% respectively. Partially regenerated plants were identified and the number of chromosomes n = 12,2 n = 24. The materials that differentiate green seedlings (Nos. 1, 10, 19, and 26) are resistant to blast, high protein content, strong cold tolerance and bacterial blight resistance. These materials provide valuable genetic resources for rice breeding.